Journal: PLOS Pathogens

Article Title: Glutamine alleviates Staphylococcus aureus -induced mastitis by modulating macrophage polarisation

doi: 10.1371/journal.ppat.1014053

Figure Lengend Snippet: RAW264.7 cells were cultured in αKG-supplemented medium, treated with the STAT3 inhibitor Stattic, and subsequently stimulated with S. aureus . A: Protein expression levels of the JAK1/STAT3 signaling pathway and its negative regulator SOCS3 were detected in the Control, αKG, and αKG + S. au groups. B-I: Relative mRNA expression of M2 and M1 marker genes across the four experimental groups. J-L: Inflammatory cytokine levels and NF-κB signaling pathway activity in the Control, αKG, αKG + S. au , and αKG + S. au +Stattic groups. M: Expression levels of the JAK1/STAT3 signaling pathway across the four experimental groups. The data are presented as mean ± SD. One-way analysis of variance was performed for statistical analysis. * P < 0.05, ** P < 0.01 and *** P < 0.001 indicate significance from each group. ns, no significance.

Article Snippet: The specific primary antibodies used in the experiment included p65 (#8242, Cell Signaling Technology, USA), p-p65 (#3033, Cell Signaling Technology, America), IκB (AF5002, Affinity Biosciences, Jiangsu, China), p-IκB (AF2002, Affinity Biosciences, Jiangsu, China), ZO-1 (AF5145, Affinity Biosciences, Jiangsu, China), Occludin (DF7504, Affinity Biosciences, Jiangsu, China), Claudin-3 (AF0129, Affinity Biosciences, Jiangsu, China), ATG5 (bsm-52596R, Bioss, Woburn, MA, USA), LC3-I/LC3-II (AF5402, Affinity Biosciences, Jiangsu, China), p62 (ab91526, Abcam plc, Cam bridge, UK), SOCS3 (bs-0580R, Bioss, Woburn, MA, USA), JAK1 (bs-1439R , Bioss, Woburn, MA, USA), p-JAK1 (bs-3238R, Bioss, Woburn, MA, USA), STAT3 (AF6294, Affinity Biosciences, Jiangsu, China), p-STAT3 (AF3293, Affinity Biosciences, Jiangsu, China) and β-actin (T0022, Affinity Biosciences, Jiangsu, China).

Techniques: Cell Culture, Expressing, Control, Marker, Activity Assay

Journal: Scientific Reports

Article Title: Cancer-Associated fibroblasts regulate the development of cholangiocarcinoma through IL-6/STAT3/AKR1C3 signaling axis

doi: 10.1038/s41598-026-37583-y

Figure Lengend Snippet: The molecular mechanism of STAT3 signaling pathway regulated by AKR1C3 in CCA. (A) After treatment with IL-6, the expression levels of proliferation, invasion, drug resistance, glycolysis, and STAT3 signaling-associated proteins were detected by Western blot in QBC939 cells. IL-6, 30 ng/ml. (B) The mRNA expression levels of the SOCSs family in QBC939 cells after AKR1C3 knockdown were detected by Q-PCR. * P < 0.05 (C) The protein expression levels of SOCS1 and SOCS3 in CCA cells after AKR1C3 knockdown and overexpression were measured by Western blot. (D) Western blot was performed to assess the ubiquitination degradation of SOCS1 in shAKR1C3- or shCtrl-transfected QBC939 cells treated with 50 µM cyclohexane (CHX) at different times. (E) Co-IP assay was conducted to investigate the protein interaction between AKR1C3 and SOCS1 in QBC939 cells.

Article Snippet: Antibodies against SOCS3 (68631 S) and PFK-1 (55028-1-AP) were obtained from Proteintech. (Hubei, China).

Techniques: Expressing, Western Blot, Knockdown, Over Expression, Ubiquitin Proteomics, Transfection, Co-Immunoprecipitation Assay

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Bioinspired cardiac-targeted metal-organic framework nanozyme for modulating inflammatory responses in heart failure with preserved ejection fraction

doi: 10.3389/fbioe.2026.1744643

Figure Lengend Snippet: Socs3 expression is elevated in HFpEF hearts and reduced after NanoAM treatment. (A,B) Volcano plots illustrate differential gene expression between groups: HF versus NC (A) , and HFi versus HF (B,C) Venn diagrams display the number of genes with opposite expression patterns in the HFi versus HF comparison relative to the HF versus NC comparison. (D) A Heatmap presents the relative expression levels of the 48 genes across three groups. (E) Interaction network between the 48 genes and insulin resistance (IR)-related genes enriched in , identifying Socs3 as the key overlapping gene.

Article Snippet: Antibodies against IRS1-Ser307 (Proteintech, 85238-1-RR, dilution 1:5000), P-AKT1-T308+AKT2-T309+AKT3-T305 (ABclonal, AP1266, dilution 1:800), SOCS3 (Proteintech, 66797-1-Ig, dilution 1:20,000), and GAPDH (Servicebio, GB15002-100, dilution 1:8000) were used.

Techniques: Expressing, Gene Expression, Comparison

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Bioinspired cardiac-targeted metal-organic framework nanozyme for modulating inflammatory responses in heart failure with preserved ejection fraction

doi: 10.3389/fbioe.2026.1744643

Figure Lengend Snippet: NanoAM regulates the SOCS3-IRS1-AKT2 axis to improve insulin resistance. (A,B) WB analysis of insulin resistance-related proteins in heart tissue (n = 6). (C) The Socs3 mRNA expression levels in heart tissues were determined by qPCR. (D) Immunofluorescence staining of GLUT4 in cardiac tissues. Scale bar, 10 µm. (E) Fluorescence intensity quantification of GLUT4 was shown. (F–I) The Inflammatory factors (IL-6,TNF-α,IL-1β,CRP) mRNA expression levels in heart tissues were determined by qPCR. (J,K) Serum levels of IL-6 and hs-CRP measured by ELISA. Data are showed as mean ± SD and analyzed using one-way ANOVA followed by Tukey’s post hoc test; *p < 0.05, ***p < 0.001.

Article Snippet: Antibodies against IRS1-Ser307 (Proteintech, 85238-1-RR, dilution 1:5000), P-AKT1-T308+AKT2-T309+AKT3-T305 (ABclonal, AP1266, dilution 1:800), SOCS3 (Proteintech, 66797-1-Ig, dilution 1:20,000), and GAPDH (Servicebio, GB15002-100, dilution 1:8000) were used.

Techniques: Expressing, Immunofluorescence, Staining, Fluorescence, Enzyme-linked Immunosorbent Assay